Kwinkqubo yokwenza umgquba, iindidi ezimbini zeentsholongwane ziyancedisa. Iseti yokuqala yeentsholongwane ezikhula phakathi kwama-20 ukuya kuma-35ºC zibizwa ngokuba zii-mesophiles. Ii-mesophiles zenza ukubola kokuqala kwaye ziphilisa umgquba kwisigaba sokugqibela senkqubo.
Iintsholongwane ze-mesophilic zikhula kwaye zivelise kwakhona ngokukhawuleza, zivelisa ubushushu ngexesha lesigaba sokuqala, ngelixa, ngexesha lokugqibela lokuvundisa, zinceda ekuvuthweni kwezinto eziphilayo ezithotyiweyo.
Phakathi kwesigaba sokuqala nesokugqibela sokwenza umgquba, kukho ukunyuka kobushushu obungundoqo bomxube wokwenza umgquba, okwaziwa njengesigaba se-thermophilic, apho ubushushu bunokwenyuka ukuya kuma-55 ukuya kuma-70ºC.
Oku kubangelwa yimveliso yobushushu ekhawulezayo kwisigaba sokuqala somgquba. I-Thermophiles inokukhula kubushushu obuphezulu kwaye incede ekwaphuleni izinto ezintsonkothileyo zezinto eziphilayo.
Ukushisa okuphezulu kunceda ekubulaweni kweentsholongwane kunye nokhula. Ukuba ne-microbial ecosystem ngundoqo ekuqinisekiseni ukuba umgquba yinkqubo esebenzayo yokulawula inkunkuma eqinileyo.